KMID : 0811720090130030209
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Korean Journal of Physiology & Pharmacology 2009 Volume.13 No. 3 p.209 ~ p.214
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Electrophysiological Characterization of AMPA and NMDA Receptors in Rat Dorsal Striatum
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Jeun Seung-Hyun
Cho Hyeong-Seok Kim Ki-Jung Li Qing-Zhong Sung Ki-Wug
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Abstract
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The striatum receives glutamatergic afferents from the cortex and thalamus, and these synaptic transmissions are mediated by ¥á-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors. The purpose of this study was to characterize glutamate receptors by analyzing NMDA/AMPA ratio and rectification of AMPA and NMDA excitatory postsynaptic currents (EPSCs) using a whole-cell voltage-clamp method in the dorsal striatum. Receptor antagonists were used to isolate receptor or subunit specific EPSC, such as (DL)-2-amino-5-phosphonovaleric acid (APV), an NMDA receptor antagonist, ifenprodil, an NR2B antagonist, CNQX, an AMPA receptor antagonist and IEM-1460, a GluR2-lacking AMPA receptor blocker. AMPA and NMDA EPSCs were recorded at ?70 and 40 mV, respectively. Rectification index was calculated by current ratio of EPSCs between £«50 and ?50 mV. NMDA/AMPA ratio was 0.20¡¾0.05, AMPA receptor ratio of GluR2-lacking/GluR2- containing subunit was 0.26¡¾0.05 and NMDA receptor ratio of NR2B/NR2A subunit was 0.32¡¾0.03. The rectification index (control 2.39¡¾0.27) was decreased in the presence of both APV and combination of APV and IEM-1460 (1.02¡¾0.11 and 0.93¡¾0.09, respectively). These results suggest that the major components of the striatal glutamate receptors are GluR2-containing AMPA receptors and NR2Acontaining NMDA receptors. Our results may provide useful information for corticostriatal synaptic transmission and plasticity studies.
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KEYWORD
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Striatum, AMPA, Glutamate receptor, NMDA, Patch clamp
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